SapphireAmp Fast PCR mix contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue) and a density reagent in 2X premix. PCR reactions with this mix can be completed in half the time required for conventional Taq DNA Polymerase amplifications. SapphireAmp is designed for fast PCR. Amplification of human genomic DNA targets of 2 kb in length can be completed in 1 hour, and amplifications of human genomic DNA targets of up to 6 kb are possible.
This product is well-suited for E.coli-based colony PCR to check for inserts of up to ~5 kb, and colony checks can be completed in about 1 hour.
SapphireAmp reactions can be assembled by simply adding primer and DNA template to the premix. Reactions performed with this mix can be loaded directly onto a gel for electrophoresis, and restriction digestion of PCR products is possible in SapphireAmp reaction buffer.
SapphireAmp Fast PCR Master Mix offers a powerful, fast, flexible and convenient alternative for both simple and complex PCR applications.
