Takara’s EmeraldAmp Max PCR Master Mix includes an optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green) and a density reagent in 2X premix. Only primer and DNA template need to be added to easily complete the reaction. The mix can be used for routine PCR applications, and PCR reactions performed with this mix can be loaded directly onto a gel for electrophoresis. This product can amplify targets up to ~10kb (genomic DNA) and will work with well on high AT or GC templates. This premix can also yield at least 10X more end product then conventional PCR and has an improved performance over standard Taq.
The EmeraldAmp Max PCR Master Mix offers a powerful, flexible and convenient alternative for high yield, simple PCR applications.
If 5 µL of the reaction mixture is used for electrophoresis with 1% Agarose L03, the blue dye marker is detected near 3~5 kb and the yellow is below 50 bp.
Those dyes have absorptions at around 260nm and 420nm, respectively, so it is recommended to remove the dye by cutting out the gel or extracting DNA by NucleoSpin Extract II.
