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Products >  PCR_Products >  PCR_Dye_Added_Premixes >  EmeraldAmp_MAX_HS_PCR_Master_Mix

EmeraldAmp MAX HS PCR Master Mix

EmeraldAmp Max HS PCR Master Mix from Takara Bio includes a high yield, Hot Start (HS) PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (green) and a density reagent in a 2X Taq PCR Master Mix format. As a result, only primers and DNA template need to be added to initiate the reaction. This Taq PCR Master Mix can be used for routine PCR applications and completed reactions can be analyzed directly via gel electrophoresis. EmeraldAmp Max HS PCR Master Mix easily amplifies targets up to ~10 kb (genomic DNA) and works well with AT- or GC-rich templates. This Taq PCR Master Mix can also yield at least 10X more product than conventional PCR and exhibits improved performance over standard Taq polymerase.

The EmeraldAmp Max HS PCR Master Mix offers a powerful, flexible and convenient option for high yield, simple PCR applications.

If 5 μL of the reaction mixture is used for electrophoresis with 1% Agarose L03, the blue dye marker is detected near 3~5 kb and the yellow dye marker is detected below 50 bp. These dyes exhibit absorbance at 260nm and 420nm, respectively; it is recommended that they be removed from the gel prior to conducting any downstream fluorescent applications. Dyes can be removed by direct cutting out of the gel or performing DNA extractions.

At-A-Glance Documents Images & Data Resources

Features

  • Antibody-mediated hot start formulation minimizes non-specific amplification and primer-dimers
  • High yield: At least 10X more end product than conventional PCR
  • Excellent performance: Optimized buffer for better performance with AT or GC-rich targets
  • Convenience: Simply add template, primers and water to the 2X Taq PCR Master Mix 
  • Reactions can be loaded directly onto a gel
  • Emerald green loading dye enables easy tracking during electrophoresis
  • Downstream restriction enzyme digests can be performed directly in the PCR buffer

Applications

  • Long PCR
  • High yield PCR

Components

(for 160 reactions, 50 µL each)

EmeraldAmp MAX HS Taq PCR Master Mix (2X Premix)4 x 1 mL
dH2O4 x 1 mL



 
 
Products
Offers Cat. # Product Package Size Price # of Units Select
RR330A EmeraldAmp® MAX HS PCR Master Mix 160 Rxns $91.00
  RR330B EmeraldAmp® MAX HS PCR Master Mix 800 Rxns $396.00
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
5003 Agarose L03 100 g $236.00
 

 


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