Takara’s EmeraldAmp GT PCR Master Mix for great performance in standard PCR applications. This master mix includes an optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green) and a density reagent in 2X premix. The EmeraldAmp GT PCR Master Mix reaction assembly can be performed by simply adding primer and DNA template to the tube of premix. Reactions performed with these mixes can be loaded directly onto an agarose gel for electrophoresis, and restriction digestion of PCR products is possible in the EmeraldAmp GT reaction buffer. This product can be used for PCR products up to ~5kb of genomic DNA and will work with templates with over 50% GC and AT content.
The EmeraldAmp GT PCR Master Mix offers a powerful, flexible and convenient alternative for simple PCR applications.
If 5 µL of the reaction mixture is used for electrophoresis with 1% Agarose L03, the blue dye marker is detected near 3~5 kb and the yellow is below 50 bp.
Those dyes have absorptions at around 260nm and 420nm, respectively, so it is recommended to remove the dye by cutting out the gel or extracting DNA by NucleoSpin Extract II.
