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Products >  PCR_Products >  High_Speed_PCR >  Z-Taq

Z-Taq

Takara Z-Taq is a novel enzyme that possesses superior properties compared to conventional Taq DNA polymerase. TaKaRa Z-Taq is characterized by 5-fold greater processivity than Taq DNA Polymerase, resulting in rapid amplification times in PCR applications. Using TaKaRa Z-Taq, a 1 kb DNA product can be amplified from genomic DNA in 20 minutes total time, using a 30-cycle, two-step amplification protocol. The improved processivity of TaKaRa Z-Taq also enables the amplification of longer templates than Taq DNA Polymerase. For example, human genomic DNA templates up to 17.5 kb, and lambda DNA templates of 20 kb have been successfully amplified.
At-A-Glance Documents Resources

Features

  • Fast PCR
  • Amplification of longer PCR products

Components

R006A

TaKaRa Z-Taq 200 U (2.5 U/µL)**
10X Z-Taq Buffer (with 30 mM MgCl2)800 µL
dNTP Mixture (2.5 mM each dNTP)800 µL
**Protocol recommends the use of 1.25 U per 50 µL reaction.

Storage

–20°C. Avoid repeated freeze-thaws of TaKaRa Z-Taq enzyme and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.

PCR Products

PCR products generated with TaKaRa Z-Taq contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency in T-vectors.

PCR Performance

Performance of rapid DNA amplification by PCR is confirmed by using a 10 kb lambda DNA template.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
R006A TaKaRa Z-Taq™ DNA Polymerase 200 Units $274.00 License Statements
R006B TaKaRa Z-Taq™ DNA Polymerase 800 Units $964.00 License Statements
 

 


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