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Products >  PCR_Products >  High_Speed_PCR >  Z-Taq

Z-Taq DNA Polymerase

TaKaRa Z-Taq DNA Polymerase has greater processivity than standard Taq DNA polymerase and allows rapid PCR amplification. Using Z-Taq polymerase, a 1 kb DNA fragment can be amplified from genomic DNA in 20 minutes total (using a 30-cycle, two-step amplification protocol). The improved processivity of Z-Taq enzyme also enables the amplification of longer fragments than standard Taq DNA polymerase; human genomic DNA templates up to 17.5 kb, and lambda DNA templates of 20 kb have been successfully amplified.

At-A-Glance Documents Images & Data Resources

Features

  • Reaction speed 5 times faster than standard Taq DNA polymerase 

Applications

  • Rapid PCR amplification

Components

R006A

TaKaRa Z-Taq 200 U (2.5 U/µL)*
10X Z-Taq Buffer (with 30 mM MgCl2)800 µL
dNTP Mixture (2.5 mM each dNTP)800 µL
*1.25 U per 50 µL reaction is recommended

Storage

–20°C. Avoid repeated freezing and thawing of TaKaRa Z-Taq enzyme and dNTPs. Once thawed, aliquot each component and store at –20°C.

PCR Products

PCR products generated with TaKaRa Z-Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency when using T-vectors.

PCR Performance

Performance is assessed by rapid PCR amplification of a 10 kb lambda DNA target.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
R006A TaKaRa Z-Taq™ DNA Polymerase 200 Units $282.00 License Statements
R006B TaKaRa Z-Taq™ DNA Polymerase 800 Units $993.00 License Statements
 

 



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