Features - Fast PCR: amplify a 2kb fragment in as little as 30 minutes
- Excellent efficiency: robust performance, comparable to high yield polymerases
- Long fragments: optimized two-buffer system allows amplification of fragments up to 20 kb with reduced optimization
- No special instrument needed: cut reaction times by two-thirds without purchasing a specialized instrument
Applications - High speed amplification cutting reaction time by two-thirds
- Robust amplification of large DNA templates
Components RR070A | | SpeedSTAR HS DNA Polymerase (5 units/µl) | 50 µL | | 10X Fast Buffer I (Mg2+*) | 1 mL | | 10X Fast Buffer II (Mg2+*) | 1 mL | | dNTP Mixture (ea. 2.5 mM) | 800 µL | *Mg2+ Concentration: 10X Fast Buffer I, 30mM; 10X Fast Buffer II, 20mM
Purity Nicking activity, endonuclease and exonuclease activity are not detected after the incubation of 0.6 µg of supercoiled pBR322, 0.6 µg of lambda DNA or 0.6 µg of lambda-Hind III digest with 10 units of this enzyme for 1 hour at 74°C.
Storage –20°C
PCR Products As most PCR products amplified with SpeedSTAR HS DNA Polymerase have one A added at 3'-terminus, the obtained PCR product can be directly used for cloning into a T-vector. It is also possible to clone the product into blunt-ended vectors after blunting and phosphorylation of the end.
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