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Products >  PCR_Products >  High_Performance_PCR >  Premix_Ex_Taq

Premix Ex Taq

Takara-brand Premix Ex Taq is an optimized 2X PCR master mix composed of TaKaRa Ex Taq enzyme, Ex Taq reaction buffer (Mg2+), and dNTP mixture. Premix Ex Taq is designed for quick and easy assembly (via dilution only) of the PCR master mix.

At-A-Glance Documents Images & Data Resources

Features

  • High yield, high sensitivity PCR
  • Premixed PCR master mix for convenient amplification requiring minimal assembly 
  • Generation of PCR products having >80% cloning efficiency in T-vectors

Components

RR003A

TaKaRa Premix Ex Taq* 6 x 500 µL

*Contains TaKaRa Ex Taq, Ex Taq Buffer, 4 mM MgCl and dNTPs.

Storage

–20°C. Avoid repeated freeze-thaw and vigorous stirring of product. Once thawed, aliquot product into PCR tubes and store at –20°C.

PCR Performance

Enzyme performance is confirmed by successful amplification of a 20 kb lambda DNA template. PCR amplification of a single copy gene (Beta-globin) by PCR is confirmed using human genomic DNA as the template (amplified fragment: 17.5 kb).

PCR Products

PCR products generated with Ex Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors.

References

1. Barnes, W.M. (1994) Proc. Natl. Acad. Sci. USA 91:2216-2220.

2. Cheng, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5695-5699.

3. Cheng, S. et al. (1994) Nature Genet. 7:350-351.

4. Cheng, S. et al. (1994) Nature 369:684-685.

5. Saiki, R.K. et al. (1988) Science 239:487-491.

6. Scharf, S.J. et al. (1986) Science 233:1076-1078.

7. Gyllensten, U.B. & Erlich, H.A. (1988) Proc. Natl. Acad. Sci. USA 85:7652-7656.

8. Kawasaki, E.S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:5698-5702.

9. Lawyer, F.C. et al. (1988) J. Biol. Chem. 264:6427-6437.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR003A Premix Taq™ DNA Polymerase (Ex Taq™ Version 2.0) 120 Rxns $158.00 License Statements
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
4030 dNTP Mixture 3.2 µmol Each/1.25 mL $57.00
 

 


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