- Convenient and reliable long range PCR
- Longer and more accurate genomic PCR amplifications
- Increased reproducibility and reduced optimization for long PCR
|One-Shot LA PCR Mix* ||24 x 25 µL|
*Contains TaKaRa LA Taq, LA PCR Buffer II (2K conc; including 5 mM Mg2+) and dNTPs.
–20°C. Avoid repeated freeze-thaws by aliquoting into separate tubes and store at –20°C.
PCR products generated with TaKaRa LA Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency into T-vectors. However, the cloning efficiency of longer inserts (>5 kb) into T-vectors is quite low.