Features - Robust amplifications of long DNA lemplates up to 48 kb possible with minimal optimization
- Longer and more accurate amplification with genomic PCR products
- High fidelity amplifications 6.5X higher than Taq DNA Polymerase
- Less optimization and greater yields due to a more robust enzyme-buffer amplification than other long polymerases.
- Reduced background and increased reaction specificity
- Multiplex PCR
Components RR042A | | TaKaRa LA Taq DNA Polymerase | 125 U (5 U/µL)* | | 10X LA PCR Buffer II (Mg2+) | 25 mM | | dNTP Mixture (2.5 mM each dNTP) | 400 µL | | *Protocol recommends the use of 2.5 U per 50 µL reaction. |
Storage –20°C. Avoid repeated freeze-thaws of TaKaRa LA Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.
PCR Products PCR products generated with TaKaRa LA Taq contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.
PCR Performance Performance is confirmed by successful amplification of a 35 kb lambda DNA template, and a 17.5 kb human genomic DNA. Inhibition of TaKaRa LA Taq activity by the antibody is confirmed to be more than 90% after an incubation of 55°C for 10 min.
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