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Products >  PCR_Products >  High_Performance_PCR >  LA_Taq_DNA_Polymerase_with_GC_Buffers

LA Taq DNA Polymerase with GC Buffers

TaKaRa LA Taq is a mixture of Taq Polymerase with a proofreading polymerase optimized for amplification of long DNA templates. Using TaKaRa LA Taq, routine extension to 20 kb is possible (and up to 48 kb on some templates), with less optimization than other long PCR systems. Because of the presence of the proofreading polymerase, fidelity is significantly better (6.5X) than Taq Polymerase alone.

The GC-optimized Buffers I and II are specifically designed to amplify DNA templates with high GC content or a significant amount of secondary structure.

GC Buffer I is recommended for amplification of fragments greater than or equal to 5 kb, whereas GC Buffer II is recommended for 2–3 kb fragments with up to 73% GC content.

At-A-Glance Documents Images & Data Resources

Features

  • Robust amplification of long DNA templates up to 48 kb possible with minimal optimization
  • Longer and more accurate amplification with genomic PCR products
  • High fidelity amplifications less optimization and greater yields due to more robust enzyme-buffer system than other long polymerases

Components

RR02AG

TaKaRa LA Taq 125 U (5 U/µL)*
2X GC Buffer I (contains 5 mM MgCl2) 1.25 mL
2X GC Buffer II (contains 5 mM MgCl2) 1.25 mL
dNTP Mixture (2.5 mM each dNTP) 400 µL
*Protocol recommends the use of 2.5 U per 50 µL reaction.

Storage

–20°C. Avoid repeated freeze-thaws of TaKaRa LA Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.

PCR Products

PCR products generated with TaKaRa LA Taq contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.

PCR Performance

Performance is confirmed by successful amplification of a 35 kb lambda DNA template, and a 17.5 kb human genomic DNA with GC Buffer I. Amplification of the c-jun proto-oncogene (1,255 bp, 65% GC content) by PCR is also confirmed using human genomic DNA as the template with GC Buffer I, II.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR02AG TaKaRa LA Taq® DNA Polymerase with GC Buffer 125 Units $136.00 License Statements
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
4030 dNTP Mixture 3.2 mol EA/1.28 mL $56.00
 

 


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