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Products >  PCR_Products >  High_Performance_PCR >  LA_Taq_DNA_Polymerase_with_GC_Buffers

A Long and Accurate PCR Enzyme for GC-Rich PCR: LA Taq DNA Polymerase with GC Buffers

TaKaRa LA Taq DNA Polymerase with GC Buffers includes buffers that allow the amplification of difficult templates. The GC-optimized Buffers I and II are specifically designed to amplify DNA templates with high GC content (GC-rich) or with a significant amount of secondary structure. GC Buffer I is recommended for amplification of fragments ≥5 kb, whereas GC Buffer II is recommended for 2–3 kb fragments with up to 73% GC content.

TaKaRa LA Taq combines Taq Polymerase and a proofreading DNA polymerase with 3′ to 5′ exonuclease activity that is optimized for long range PCR. This mixture of enzymes allows for long and accurate (LA) PCR amplification of targets from a variety of templates, including genomic DNA. The presence of the proofreading polymerase increases fidelity (6.5X) compared to Taq Polymerase alone. In combination with the GC Buffers, LA Taq can be used for amplication of targets from difficult-to-amplify templates.

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Features

  • Robust enzyme-buffer system for difficult templates –optimized buffers (GC Buffers I & II) for amplification of templates with high GC content and/or significant secondary structure
  • High yield amplification of long DNA targets up to 48 kb is possible with minimal optimization
  • Long and accurate PCR amplification of genomic DNA targets (long range PCR)

Components

RR02AG

TaKaRa LA Taq 125 U (5 U/µL)*
2X GC Buffer I (contains 5 mM MgCl2) 1.25 mL
2X GC Buffer II (contains 5 mM MgCl2) 1.25 mL
dNTP Mixture (2.5 mM each dNTP) 400 µL
*Protocol recommends the use of 2.5 U per 50 µL reaction.

Storage

–20°C. Avoid repeatedly freezing and thawing TaKaRa LA Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.

PCR Products

PCR products generated with TaKaRa LA Taq contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.

PCR Performance

Performance is confirmed by successful amplification of a 35 kb lambda DNA template, and a 17.5 kb human genomic DNA with GC Buffer I. Amplification of the c-jun proto-oncogene (1,255 bp, 65% GC content) by PCR is also confirmed using human genomic DNA as the template with GC Buffer I and II.




 
 
Products
Offers Cat. # Product Contents Size Price License Units Select
RR02AG TaKaRa LA Taq® DNA Polymerase with GC Buffer 125 Units $140.00 License Statements
 

 

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