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Products >  PCR_Products >  High_Performance_PCR >  LA_Taq_DNA_Polymerase

A High Fidelity PCR Enzyme for Long Range PCR: LA Taq DNA Polymerase

TaKaRa LA Taq combines Taq DNA Polymerase and a DNA proofreading polymerase with 3’ to 5’ exonuclease activity that is optimized for PCR amplification of very long DNA templates (long range PCR). This mixture of enzymes allows for long and accurate (LA) PCR amplification of targets from a variety of templates, including genomic DNA. The presence of the proofreading polymerase significantly increases fidelity (6.5X) as compared to Taq polymerase alone. Using the TaKaRa LA Taq long PCR system, routine extension of 20 kb is possible, and products up to 48 kb can be obtained for some templates.

At-A-Glance Documents Images & Data Resources

Features

  • Robust amplification of long DNA templates (long range PCR) – obtaining products up to 48 kb is possible with minimal reaction optimization
  • Accurate, long PCR amplification of genomic targets
  • DNA proofreading activity allows for high fidelity amplification from a variety of templates
  • High yields are possible with the robust enzyme-buffer system – includes an optimized buffer (LA Buffer II)

Components

RR002A

TaKaRa LA Taq DNA Polymerase 125 U (5 U/µL)*
10X LA PCR Buffer II (without Mg2+)
25 mM MgCl2
1 mL
1 mL
dNTP Mixture (2.5 mM each dNTP) 400 µL
 

RR002M

TaKaRa LA Taq DNA Polymerase 250 U (5 U/µL)*
10X LA PCR Buffer II (contains 25 mM MgCl2) 1 mL
dNTP Mixture (2.5 mM each dNTP) 800 µL


*Protocol recommends the use of 2.5 U per 50 µL reaction.

Storage

Avoid repeatedly freezing and thawing TaKaRa LA Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.

Products

PCR products generated with LA Taq contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.


PCR Performance

Enzyme performance is confirmed by successful amplification of a 35 kb lambda DNA template and a 17.5 kb human genomic DNA template.




 
 
Products
Offers Cat. # Product Package Size Price License # of Units Select
RR002A TaKaRa LA Taq® DNA Polymerase (Mg2+ free buffer) 125 Units $140.00 License Statements
  RR002M TaKaRa LA Taq® DNA Polymerase (Mg2+ plus buffer) 250 Units $282.00 License Statements
  RR002C TaKaRa LA Taq® DNA Polymerase (Mg2+ plus buffer) 3,000 Units $2,979.00 License Statements
  RR002B TaKaRa LA Taq® DNA Polymerase (Mg2+ plus buffer) 1,000 Units $1,049.00 License Statements
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
4030 dNTP Mixture 3.2 µmol Each/1.25 mL $57.00
 

 


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