Features - Amplification of Large DNA Templates: 30 kb genomic or 48 kb lambda DNA
- Longer and More Accurate Genomic PCR Products
- GC Buffers at 2X Concentration Included: for templates with high GC-content or strong secondary structures
Components RR013A | | TaKaRa LA Taq | 125 U (5 U/µL) | | 10X LA PCR Buffer II (contains 25 mM Mg2+) | 250 µL | | 10X LA PCR Buffer II (without Mg2+) | 250 µL | | MgCl2 (25 mM) | 500 µL | | dNTP Mixture (2.5 mM each dNTP) | 400 µL | | Control Template (100 ng/µL HT29 DNA) | 10 µL | | Control Primer LA3 (10 pmol/µL)* | 10 µL | | Control Primer LA4 (10 pmol/µL)* | 10 µL | | Lambda-Hind III MW Markers (100 ng/µL) | 20 µL | | 2X GC Buffer I (contains 5 mM MgCl2) | 1.25 mL | | 2X GC Buffer II (contains 5 mM MgCl2) | 1.25 mL | | Control Primer GC1 (10 pmol/µL)** | 10 µL | | Control Primer GC2 (10 pmol/µL)** | 10 µL | * Amplifies a 17.5 kb region of the Control Template.
** Amplifies a 1,255 bp GC-rich region of the Control Template.
Storage –20°C
PCR Properties PCR products generated with TaKaRa LA Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends which give >80% cloning efficiency in T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.
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