Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
PCR Products


Products >  PCR_Products >  High_Performance_PCR >  Ex_Taq_DNA_Polymerase_Hot-Start

Ex Taq DNA Polymerase, Hot-Start Version

TaKaRa Ex Taq HS DNA Polymerase offers the same high performance as the standard TaKaRa Ex Taq polymerase including high yield, excellent sensitivity, and fidelity 4.5X better than Taq Polymerase, along with the advantages of hot-start: lower background, increased specificity, and room temperature reaction assembly.

TaKaRa Ex Taq HS DNA Polymerase includes a monoclonal antibody to Taq DNA Polymerase that binds to the polymerase and prevents nonspecific amplification due to mispriming and/or formation of primer dimers during reaction assembly. The antibody is then denatured during the initial PCR DNA-denaturation step, releasing the polymerase and allowing DNA synthesis to proceed.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • High yield and high sensitivity PCR
  • Excellent on difficult templates
  • Robust polymerase
  • Antibody mediated hot-start: lower background, increased specificity, and room temperature reaction assembly

Applications

  • Robust, specific amplification with reduced background
  • Amplifications requiring room-temperature reaction assembly

Components

RR006A

TaKaRa Ex Taq HS 250 U (5 U/µL)*
10X Ex Taq Buffer (contains 20 mM MgCl2)1 mL
dNTP Mixture (2.5 mM each dNTP)800 µL

*Protocol recommends use of 1.25 U per 50 µL reaction.

RR030A Premix Ex Taq Hot Start Version

TaKaRa Ex Taq HS, Premix**5 x 500µL

**Contains TaKaRa Ex Taq HS, 1.25 units/25 µL; dNTP Mixture, 2X conc.; ea. 0.4 mM Ex Taq Buffer, 2X conc.; including 4 nM Mg2+.

Storage

–20°C. Avoid repeated freeze-thaws and vigorous stirring. Once thawed, aliquot into tubes and store at –20°C.

PCR Products

PCR products generated with TaKaRa Ex Taq HS contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency in T-vectors.

PCR Performance

Enzyme performance is confirmed by successful amplification of a 20 kb lambda DNA template. Amplification of a single copy gene (beta-globin) by PCR is also confirmed using human genomic DNA as the template (amplified fragment: 17.5 kb). Inhibition of TaKaRa Ex Taq activity by the antibody is confirmed to be more than 90% after an incubation at 55°C for 10 min.




 
 
Products
Offers Cat. # Product Contents Size Price License Units Select
  RR030A Premix Ex Taq™ DNA Polymerase Hot Start Version 100 Rxns $161.00 License Statements
RR006A TaKaRa Ex Taq® DNA Polymerase Hot-Start Version 250 Units $203.00 License Statements
  RR006B TaKaRa Ex Taq® DNA Polymerase Hot-Start Version 1,000 Units $714.00 License Statements
 

 

Recommended Products
Offers Cat. # Product Contents Size Price Units Select
  4030 dNTP Mixture 3.2 µmol Each/1.25 mL $57.00
9002A Taq Antibody 250 Units $110.00
 

 

 
 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions