Ex Taq DNA Polymerase: A Robust PCR Enzyme with Proofreading Activity
TaKaRa Ex Taq DNA Polymerase combines the proven performance of TaKaRa Taq polymerase with the proofreading activity of an efficient 3' to 5' exonuclease for high sensitivity, high efficiency PCR reactions. In routine PCR applications, using the Ex Taq polymerase and Ex Taq Buffer system results in higher yields of PCR products as compared to standard Taq DNA polymerase. In addition, Ex Taq polymerase has a higher fidelity that standard Taq (mutation rate approximately 4.5X lower, as determined by the Kunkel method). Ex Taq DNA Polymerase also can be used for long range PCR; high yields of long products, up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates, are possible with this enzyme.