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Products >  PCR_Products >  High_Performance_PCR >  Ex_Taq_DNA_Polymerase

Ex Taq DNA Polymerase: A Robust PCR Enzyme with Proofreading Activity

TaKaRa Ex Taq DNA Polymerase combines the proven performance of TaKaRa Taq polymerase with the proofreading activity of an efficient 3' to 5' exonuclease for high sensitivity, high efficiency PCR reactions. In routine PCR applications, using the Ex Taq polymerase and Ex Taq Buffer system results in higher yields of PCR products as compared to standard Taq DNA polymerase. In addition, Ex Taq polymerase has a higher fidelity that standard Taq (mutation rate approximately 4.5X lower, as determined by the Kunkel method). Ex Taq DNA Polymerase also can be used for long range PCR; high yields of long products, up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates, are possible with this enzyme.

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Features

  • First choice for high fidelity, high yield endpoint PCR
  • A wide range of PCR products can be obtained (<100 bp to 20 kb for genomic DNA targets)
  • Less optimization and more reproducible results than Taq – optimized 10X Buffer and dNTPs are included with the enzyme
  • PCR products can be obtained for a wide variety of templates, including genomic DNA and plant samples
  • DNA proofreading activity allows for high fidelity and high sensitivity reactions
  • A high performance PCR enzyme that can amplify even difficult templates
  • Components

    RR001A

    TaKaRa Ex Taq DNA Polymerase 250 U (5 U/µL)*
    10X Ex Taq Buffer (contains 20 mM MgCl2) 1 mL
    dNTP Mixture (2.5 mM each dNTP) 800 µL
     

    RR01AM

    TaKaRa Ex Taq DNA Polymerase 250 U (5 U/µL)*
    10X Ex Taq Buffer (without MgCl2) 1 mL
    25 mM MgCl2 1 mL
    dNTP Mixture (2.5 mM each dNTP) 800 µL
    *Protocol recommends the use of 1.25 U per 50 µL reaction.

    Purity

    Nicking activity, endonuclease and exonuclease activity are not detected after the incubation of 0.6 µg of supercoiled pBR322 DNA, 0.6 mg of lambda DNA, or 0.6 µg of lambda-Hind III digest with 10 units of this enzyme for 1 hour at 74°C.

    Storage

    –20°C

    Concentration

    5 units/µL

    Form

    20 mM Tris HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 50% Glycerol

    PCR Products

    Most PCR products (approximately 80%) amplified with TaKaRa Ex Taq have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for cloning into T-vectors (TA cloning). It is possible to clone the product into blunt-end vectors after blunting and phosphorylation.

    PCR Performance Test

    PCR performance is confirmed by using lambda DNA as the template (amplified fragment: 20 kb).

    PCR performance is also confirmed via amplification of the beta globin gene from human DNA template (amplified fragment: 17.5 kb).




     
     
    Products
    Offers Cat. # Product Contents Size Price License Units Select
    RR001A TaKaRa Ex Taq® DNA Polymerase 250 Units $169.00 License Statements
      RR001B TaKaRa Ex Taq® DNA Polymerase 1,000 Units $608.00 License Statements
      RR001C TaKaRa Ex Taq® DNA Polymerase 3,000 Units $1,588.00 License Statements
      RR01AM TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer) 250 Units $169.00 License Statements
      RR01CM TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer) 3,000 Units $1,588.00 License Statements
      RR01BM TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer) 1,000 Units $608.00 License Statements
     

     

     
     

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