FeaturesFirst choice for high fidelity, high yield endpoint PCRA wide range of PCR products can be obtained (<100 bp to 20 kb for genomic DNA targets)Less optimization and more reproducible results than Taq – optimized 10X Buffer and dNTPs are included with the enzymePCR products can be obtained for a wide variety of templates, including genomic DNA and plant samplesDNA proofreading activity allows for high fidelity and high sensitivity reactionsA high performance PCR enzyme that can amplify even difficult templates
Components RR001A | | TaKaRa Ex Taq DNA Polymerase | 250 U (5 U/µL)* | | 10X Ex Taq Buffer (contains 20 mM MgCl2) | 1 mL | | dNTP Mixture (2.5 mM each dNTP) | 800 µL | | | RR01AM | | TaKaRa Ex Taq DNA Polymerase | 250 U (5 U/µL)* | | 10X Ex Taq Buffer (without MgCl2) | 1 mL | | 25 mM MgCl2 | 1 mL | | dNTP Mixture (2.5 mM each dNTP) | 800 µL | | *Protocol recommends the use of 1.25 U per 50 µL reaction. |
Purity Nicking activity, endonuclease and exonuclease activity are not detected after the incubation of 0.6 µg of supercoiled pBR322 DNA, 0.6 mg of lambda DNA, or 0.6 µg of lambda-Hind III digest with 10 units of this enzyme for 1 hour at 74°C.
Storage –20°C
Concentration 5 units/µL
Form 20 mM Tris HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 50% Glycerol
PCR Products Most PCR products (approximately 80%) amplified with TaKaRa Ex Taq have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for cloning into T-vectors (TA cloning). It is possible to clone the product into blunt-end vectors after blunting and phosphorylation.
PCR Performance Test PCR performance is confirmed by using lambda DNA as the template (amplified fragment: 20 kb). PCR performance is also confirmed via amplification of the beta globin gene from human DNA template (amplified fragment: 17.5 kb).
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