Features - High yield and high sensitivity PCR
- Excellent on difficult templates
- Robust polymerase
- Reproducible PCR results
- Improved fidelity
Components RR001A | | TaKaRa Ex Taq DNA Polymerase | 250 U (5 U/µL)* | | 10X Ex Taq Buffer (contains 20 mM MgCl2) | 1 mL | | dNTP Mixture (2.5 mM each dNTP) | 800 µL | | | RR01AM | | TaKaRa Ex Taq DNA Polymerase | 250 U (5 U/µL)* | | 10X Ex Taq Buffer (without MgCl2) | 1 mL | | 25 mM MgCl2 | 1 mL | | dNTP Mixture (2.5 mM each dNTP) | 800 µL | | *Protocol recommends the use of 1.25 U per 50 µL reaction. |
Purity Nicking activity, endonuclease and exonuclease activity are not detected after the incubation of 0.6 µg of supercoiled pBR322 DNA, 0.6 mg of lambda DNA or 0.6 µg of lambda-Hind III digest with 10 units of this enzyme for 1 hour at 74°C.
Storage –20°C
Concentration 5 units/µL
Form 20 mM Tris HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 50% Glycerol
PCR Products As most PCR products amplified with TaKaRa Ex Taq have one A added at the 3'-termini, the obtained PCR product can be directly used for cloning into T-vector. Also it is possible to clone the product into blunt-end vectors after blunting and phosphorylation of the end. Approximately 80% of the clones have a 3' A overhang.
PCR Performance Test PCR performance by Polymerase Chain Reaction (PCR) is confirmed by using lambda DNA as the template (amplified fragment: 20 kb). PCR performance is also confirmed via amplification of the beta globin gene from a human DNA template (amplified fragment: 17.5 kb).
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