PrimeSTAR HS with GC Buffer was developed for high-fidelity amplification of high GC (greater than or equal to 75%) templates. PrimeSTAR HS is a unique high fidelity DNA polymerase, offering both maximal accuracy and higher amplification efficiency than Taq Polymerase.
The new GC buffer formulation facilitates robust extension through even very high GC regions efficiently and accurately. Inclusion of a monoclonal antibody suppresses both the DNA polymerase and 3'-->5' exonuclease activities prior to the first denaturing step, preventing false initiation events during reaction assembly and primer digestion. PrimeSTAR HS with GC buffer provides reliable amplification, high accuracy and high specificity in applications where amplification of high-GC DNA templates for cloning or library construction is required.
