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Products >  PCR_Products >  High_Fidelity_PCR >  PrimeSTAR_HS_with_GC_Buffer

PrimeSTAR HS with GC Buffer

PrimeSTAR HS with GC Buffer is intended for high fidelity PCR amplification of GC-rich template that contains 75% or greater GC content. PrimeSTAR HS with GC Buffer is a unique, high fidelity PCR polymerase, offering both maximal accuracy and better amplification efficiency of high GC content PCR than regular Taq polymerase.

The GC buffer that is supplied with PrimeSTAR HS facilitates robust, efficient and accurate extension through even highly GC-rich template regions. Inclusion of a monoclonal antibody suppresses both the DNA polymerase and 3'-->5' exonuclease activities of PrimeSTAR HS polymerase prior to the first denaturation step. This prevents false initiation events during reaction assembly and primer digestion. PrimeSTAR HS with GC buffer provides reliable amplification and exceptional accuracy and specificity in high GC content PCR applications where amplification of high GC DNA templates for cloning or library construction is required.

At-A-Glance Documents Images & Data Resources

Features

  • Only 25 errors per 304 kb when using GC-rich template DNA (as determined by sequence analysis)
  • Higher efficiency than standard Taq polymerase
  • Capable of amplifying high-GC human genomic DNA targets up to 5 kb
  • Fast reaction time due to the increased priming efficiency
  • High specificity due to the antibody-mediated hot-start technology

Applications

  • High fidelity PCR
  • High GC content PCR
  • Amplification of cDNA libraries
  • cDNA cloning for protein expression

Components

R044A

PrimeSTAR HS DNA Polymerase (2.5 U/µL)100 µL
2X PrimeSTAR GC Buffer (Mg2+)*1.7 mL
dNTP Mixture (2.5 mM each)800 µL

*Mg2+ concentration is 2 mM (2X)

Purity

Nicking, endonuclease and exonuclease activities are not detected upon incubation of 0.6 µg of supercoiled or lambda-Hind II digested pBR322 DNA with 10 units of PrimeSTAR HS enzyme for 1 hour at 74°C.

Storage

–20°C.

Storage Buffer: [50 mM Tris-HCL (pH 8.2 at 4°C), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween 20, 0.1% Nonidet P-40, 50% Glycerol]




 
 
Products
Cat. # Product Package Size Price License # of Units Select
R044A PrimeSTAR® HS DNA Polymerase with GC Buffer 250 Units $185.00 License Statements
R044B PrimeSTAR® HS DNA Polymerase with GC Buffer 1,000 Units $618.00 License Statements
 

 



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