PrimeSTAR HS DNA Polymerase is a novel high fidelity PCR enzyme which provides maximum fidelity as well as extended product length (8.5 kb for human genomic DNA; 22 kb for lambda DNA). Targeted for demanding cloning (i.e., amplification of cDNA libraries) and sequencing applications, it offers extremely high accuracy, and is the only enzyme whose fidelity is calculated by direct sequence analysis. Sequence analysis provides a more realistic estimate of the actual frequency of errors than other commonly-used indirect phenotypic analysis (i.e., lacl,) used by other suppliers and which are prone to error due to lethal and silent mutations.
It also offers excellent amplification efficiency and shortened reaction times. Finally, the antibody-mediated hot-start formulation prevents false initiation events during reaction assembly due to mispriming or primer digestion, lowering background.
PrimeSTAR HS DNA Polymerase possesses extremely high priming efficiency. Therefore, highly specific amplification can be achieved using a short annealing time, only 5-15 seconds.
PrimeSTAR HS (Premix): PrimeSTAR HS (Premix) is an optimized mixture composed of PrimeSTAR HS DNA Polymerase, a high-fidelity DNA polymerase, reaction buffer and dNTP mixture as 2-fold concentration. This is an excellent product for high-throughput applications because of the premixed reaction mixture, which allows reduced contamination risk.
Note regarding fidelity comparisons: Takara has chosen to use sequence analysis for determination of error rate because most researchers who require high fidelity perform a series of reactions: PCR, cloning and sequencing. Determination of error rate by direct sequence analysis, therefore, corresponds most closely to the rate which researchers would expect to observe during their own applications. Please note that values obtained using different fidelity measurement methods (i.e,. direct sequencing, Kunkel method, Cline method, lacI method, etc.) do not yield directly comparable results.
