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Products >  PCR_Products >  General_PCR_Products >  Taq_and_Premix_Taq

TaKaRa Taq and Premix Taq

TaKaRa Taq DNA Polymerase is a recombinant and thermostable 94 kDa Taq polymerase encoded by a DNA polymerase gene derived from the Thermus aquaticus YT-1 strain and cloned into Escherichia coli. TaKaRa Taq has been demonstrated to have the same characteristics and capabilities as native Taq polymerase.

TaKaRa Taq is a versatile and thermostable DNA polymerase suitable for a variety of standard PCR applications. The enzyme is also supplied as a Premix Taq (Cat. #R004A), meaning that the item is offered as a 2X PCR master mix containing Taq polymerase enzyme, buffer and dNTPs. The Premix Taq formulation of TaKaRa Taq simplifies PCR setup and minimizes pipetting steps. This is particularly helpful for saving time during high-throughput screening projects. 
At-A-Glance Documents Images & Data Resources

Features

  • Excellent for standard PCR amplifications
  • Low bacterial DNA contamination enzyme
  • Premix Taq enables easy PCR setup and minimizes pipetting steps

Applications

  • Routine PCR
  • DNA sequencing

Components

R001A

TaKaRa Taq250 U (5 U/µL)*
10X PCR Buffer (contains 15 mM MgCl2)1 mL
dNTP Mixture (2.5 mM each dNTP)800 µL
 

R001AM

TaKaRa Taq250 U (5 U/µL)*
10X PCR Buffer (without Mg2+)1 mL
25 mM MgCl2 1 mL
dNTP Mixture (2.5 mM each dNTP)800 µL
 

R004A - Premix Taq (TaKaRa Taq Version)

2X Taq Polymerase Premix 6 x 500 µL**

*Protocol recommends the use of 1.25 U per 50 µL reaction.
**Contains TaKaRa Taq, PCR Buffer and dNTPs.

Source

Recombinant E. coli

Storage

–20°C. Avoid repeated freeze-thaw of TaKaRa Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.

PCR Performance

Enzymatic performance is confirmed by successful amplification of an 8 kb lambda DNA template. PCR amplification of a single copy gene is also confirmed using a 2.9 kb human genomic DNA template and human p53 primers.


 
 
Products
Offers Cat. # Product Package Size Price License # of Units Select
  R004A Premix Taq™ DNA Polymerase (TaKaRa Taq™ Version 2.0) 150 Units $128.00 License Statements
R001A TaKaRa Taq™ DNA Polymerase 250 Units $118.00 License Statements
  R001B TaKaRa Taq™ DNA Polymerase 1,000 Units $407.00 License Statements
  R001C TaKaRa Taq™ DNA Polymerase 3,000 Units $1,071.00 License Statements
  R001AM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 250 Units $118.00 License Statements
  R001CM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 3,000 Units $1,071.00 License Statements
  R001BM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 1,000 Units $407.00 License Statements
 

 

Recommended Products
Offers Cat. # Product Package Size Price # of Units Select
4030 dNTP Mixture 3.2 µmol Each/1.25 mL $57.00
 

 


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