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TaKaRa Taq DNA Polymerase and Premix Taq DNA Polymerase

TaKaRa Taq DNA Polymerase is a versatile and thermostable polymerase suitable for a variety of standard PCR applications. The enzyme is also supplied as a Premix Taq DNA polymerase (Cat. #R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs. This premix formulation simplifies PCR setup and minimizes pipetting steps. This is particularly helpful for high-throughput screening projects.

TaKaRa Taq DNA Polymerase is a recombinant and thermostable 94 kDa Taq polymerase encoded by a DNA polymerase gene derived from the Thermus aquaticus YT-1 strain and cloned into Escherichia coli. TaKaRa Taq DNA Polymerase has been demonstrated to have the same characteristics and capabilities as native Taq DNA polymerase.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Excellent for standard PCR amplifications
  • Low bacterial DNA contamination enzyme
  • Premix Taq enables easy PCR setup and minimizes pipetting steps

Applications

  • Routine PCR
  • DNA sequencing

Components

R001A

TaKaRa Taq250 U (5 U/µL)*
10X PCR Buffer (contains 15 mM MgCl2)1 mL
dNTP Mixture (2.5 mM each dNTP)800 µL
 

R001AM

TaKaRa Taq250 U (5 U/µL)*
10X PCR Buffer (without Mg2+)1 mL
25 mM MgCl2 1 mL
dNTP Mixture (2.5 mM each dNTP)800 µL
 

R004A - Premix Taq (TaKaRa Taq Version)

2X Taq Polymerase Premix 6 x 500 µL**

*Protocol recommends the use of 1.25 U per 50 µL reaction.
**Contains TaKaRa Taq, PCR Buffer and dNTPs.

Source

Recombinant E. coli

Storage

–20°C. Avoid repeated freeze-thaw of TaKaRa Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.

PCR Performance

Enzymatic performance is confirmed by successful amplification of an 8 kb lambda DNA template. PCR amplification of a single copy gene is also confirmed using a 2.9 kb human genomic DNA template and human p53 primers.




 
 
Products
Offers Cat. # Product Contents Size Price License Units Select
  R004A Premix Taq™ DNA Polymerase (TaKaRa Taq™ Version 2.0) 150 Units $132.00 License Statements
R001A TaKaRa Taq™ DNA Polymerase 250 Units $122.00 License Statements
R001B TaKaRa Taq™ DNA Polymerase 1,000 Units $419.00 License Statements
R001C TaKaRa Taq™ DNA Polymerase 3,000 Units $1,103.00 License Statements
R001AM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 250 Units $122.00 License Statements
R001CM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 3,000 Units $1,103.00 License Statements
R001BM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 1,000 Units $419.00 License Statements
 

 

Recommended Products
Cat. # Product Contents Size Price Units Select
4030 dNTP Mixture 3.2 µmol Each/1.25 mL $57.00
 

 

 
 

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