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Products >  PCR_Products >  General_PCR_Products >  Taq_and_Premix_Taq

Taq and Premix Taq

Takara Taq is a recombinant, thermostable, 94 kDa DNA polymerase encoded by the DNA polymerase gene of the Thermus aquaticus YT-1 strain which has been cloned into Escherichia coli. It has been shown to have essentially the same characteristics as native Taq DNA polymerase.

TaKaRa Taq is a versatile thermostable DNA polymerase suitable for a variety of standard PCR applications. The enzyme is also supplied in a premix format (a mixture of enzyme, buffer, and dNTPs) which simplifies the setup of PCR reactions and minimizes pipetting steps.
At-A-Glance Documents Images & Data Resources

Features

  • Excellent for standard PCR amplifications
  • Low DNA contamination enzyme
  • Premix format allows for easier set up and minimizes pipetting steps

Applications

  • Routine PCR
  • Thermal cycle sequencing

Components

R001A

TaKaRa Taq 250 U (5 U/µL)*
10X PCR Buffer (contains 15 mM MgCl2) 1 mL
dNTP Mixture (2.5 mM each dNTP) 800 µL
 

R001AM

TaKaRa Taq 250 U (5 U/µL)*
10X PCR Buffer (without Mg2+) 1 mL
25 mM MgCl2 1 mL
dNTP Mixture (2.5 mM each dNTP) 800 µL
 

R004A - Premix Taq (TaKaRa Taq Version)

2X Taq Polymerase Premix 6 x 500 µL**

*Protocol recommends the use of 1.25 U per 50 µL reaction.
**Contains TaKaRa Taq, PCR Buffer and dNTPs.

Source

Recombinant E. coli

Storage

–20°C. Avoid repeated freeze-thaws of TaKaRa Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.

PCR Performance

Enzyme performance is confirmed by successful amplification of an 8 kb lambda DNA template. Amplification of a single copy gene by PCR is also confirmed using a 2.9 kb human genomic DNA template and human p53-primers.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
R004A Premix Taq™ DNA Polymerase (TaKaRa Taq™ Version 2.0) 150 Units $128.00 License Statements
R001A TaKaRa Taq™ DNA Polymerase 250 Units $118.00 License Statements
R001B TaKaRa Taq™ DNA Polymerase 1,000 Units $407.00 License Statements
R001C TaKaRa Taq™ DNA Polymerase 3,000 Units $1,071.00 License Statements
R001AM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 250 Units $118.00 License Statements
R001CM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 3,000 Units $1,071.00 License Statements
R001BM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 1,000 Units $407.00 License Statements
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
4030 dNTP Mixture 3.2 mol EA/1.28 mL $56.00
 

 


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