TaKaRa Taq DNA Polymerase is a hot start PCR enzyme that contains a mixture of Taq polymerase and monoclonal antibody to Taq polymerase; this monoclonal antibody binds the Taq polymerase until reaction temperature is elevated to DNA denaturation level (~94°C). At that point, the antibody is denatured and releases TaKaRa Taq, allowing DNA synthesis to proceed. The use of a hot start PCR enzyme such as TaKaRa Taq prevents nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly.
Premix Taq DNA Polymerase, Hot Start Version
Premix Taq DNA Polymerase, Hot Start Version is an optimized mixture composed of 2X TaKaRa Taq and optimized reaction buffer containing dNTPs.