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Products >  PCR_Products >  General_PCR_Products >  Taq_Hot-Start_Version

Hot Start PCR Enzyme: Hot Start TaKaRa Taq DNA Polymerase

These hot start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody to Taq polymerase. The antibody binds Taq polymerase, thereby preventing DNA synthesis. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot start PCR enzyme prevents nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly.

Several hot start versions of TaKaRa Taq are available:

TaKaRa Taq DNA Polymerase Hot Start Version: enzyme, buffer, and dNTPs are supplied as separate components.

Premix Taq DNA Polymerase Hot Start Version: 2X premix containing TaKaRa Taq HS enzyme, dNTPs, and an optimized reaction buffer.

TaKaRa Taq HS Perfect Mix: 2X premix optimized for fast reactions; compatible with a 20 sec./kb extension step.

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Features

  • All hot start TaKaRa Taq versions contain an anti-Taq polymerase monoclonal antibody that improves specificity, reduces background, and allows room temperature assembly
  • TaKaRa Taq DNA Polymerase Hot Start Version: enzyme, buffer, and dNTPs are supplied as separate components.
  • Premix Taq DNA Polymerase Hot Start Version: 2X premix containing TaKaRa Taq HS enzyme, dNTPs, and an optimized reaction buffer.
  • TaKaRa Taq HS Perfect Mix: 2X premix optimized for fast reactions; compatible with a 20 sec./kb extension step.

Applications

  • Hot start PCR
  • Multiplex PCR

Components

TaKaRa Taq DNA Polymerase, Hot Start Version (Cat. #R007A) 

TaKaRa Taq  250 U (5 U/µL)*
10X PCR Buffer (contains 15 mM MgCl2)1 mL
dNTP Mixture (2.5 mM each dNTP)800 µL
 * Use 1.25 U per 50 µL reaction.

Premix Taq DNA Polymerase, Hot Start Version (Cat. # R028A) 

2X TaKaRa Taq, Premix*5 X 500 µL
 * 2X dNTP Mixture (0.4 mM each) and 2X PCR buffer (20 mM Tris-HCl (pH 8.3), 100 mM KCI, and 3 mM MgCl2)

TaKaRa Taq Perfect Mix (Cat. # R300A)

 
TaKaRa Taq HS Perfect Mix (2X) 5 X 500 µL     

Storage

–20°C. Avoid repeated freezing and thawing.




 
 
Products
New Offers Cat. # Product Contents Size Price License Units Select
    R028A Premix Taq™ DNA Polymerase Hot-Start Version 100 Rxns $129.00 License Statements
  R007A TaKaRa Taq™ DNA Polymerase Hot Start Version 250 Units $138.00 License Statements
  R007B TaKaRa Taq™ DNA Polymerase Hot Start Version 1,000 Units $497.00 License Statements
  R300A TaKaRa Taq™ HS Perfect Mix 100 Rxns $99.00  
  R300B TaKaRa Taq™ HS Perfect Mix 400 Rxns $396.00  
 

 

 
 

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