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Products >  PCR_Products >  General_PCR_Products >  Taq_Antibody

Taq Antibody

Taq antibody is a monoclonal antibody that binds Taq Polymerase and inhibits its activity until reaction temperature is elevated. At that point, the Taq antibody is denatured and releases its hold on Taq polymerase, allowing DNA synthesis to proceed. 

The binding of the Taq antibody to Taq polymerase is the premise of hot start PCR. By preventing Taq polymerase activity until the dentauration step of PCR is underway, nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly is prevented. As a result, experimental background is significantly decreased and reaction specificity is increased.

The Taq antibody can be added to Taq Polymerase, TaKaRa Ex Taq or TaKaRa LA Taq before PCR to lower background, increase specificity and allow room temperature reaction assembly.

At-A-Glance Documents Images & Data

Applications

  • Hot start PCR

 

Storage

–20°C

Concentration

5 U/µL

Antibody Performance

Following incubation of Taq antibody and Taq polymerase at 25°C for 10 min, inhibition of Taq polymerase activity was confirmed as greater than 90% (at 55°C for 10 min).

Following heat treatment at 94°C for 30 seconds, Taq antibody-bound Taq polymerase was confirmed to recover almost 100% activity (at 55°C for 10 minutes).


 
 
Products
Cat. # Product Package Size Price License # of Units Select
9002A Taq Antibody 250 Units $110.00 License Statements
9002B Taq Antibody 1,000 Units $354.00 License Statements
 

 


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