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Products >  PCR_Products >  General_PCR_Products >  PCR_Mycoplasma_Detection_Set

PCR Mycoplasma Detection Set

The PCR Mycoplasma Detection Set is a primer set designed to detect the presence of mycoplasma in biological materials such as cultured cells. Nested PCR is performed first using primers hybridizing to highly conserved ribosomal genes (16S and 23S rRNA), and then using primers hybridizing to the species-specific spacer region between these ribosomal genes. Species verification is determined by gel electrophoresis of the amplified products. The PCR Mycoplasma Detection Set allows rapid assessment of cell culture contamination, shortening turnaround time from about one week using conventional methods to a few hours. This kit allows the highly sensitive and specific detection of the following Mycoplasma species: M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. neurolyticum, M. hypopneumoniae, M. capricolum, and Ureaplasma urealyticum.

Product Amplification Size
1st PCR (bp) 2nd PCR (bp)
M. hyopneumoniae 681 237
M. neurolyticum 501 196
M. fermentans 491 195
M. pulmonis 477 189
M. hyorhinis 448 211
M. orale 423 211
M. capricolum 415 179
M. arthritidis 408 157
M. salivarium 403 151
M. hominis 370, 369 147, 148
M. arginini 369 145
U. urealyticum 482, 481 154
At-A-Glance Documents Images & Data Resources

Features

  • Rapid detection of the Mycoplasma species using nested PCR
  • Fast turnaround: save time by determining assay outcome in several hours vs one week
  • High sensitivity and specificity detection
  • Control template included

Applications

  • Rapid detection, via nested PCR, of the Mycoplasma species most frequently found to contaminate cell cultures

Components

6601

MCGp F1 Primer (20 pmol/µL) 50 µL
MCGp R1 Primer (20 pmol/µL) 50 µL
MCGp F2 Primer (20 pmol/µL) 50 µL
MCGp R2 Primer (20 pmol/µL) 50 µL
Control Template (1 ng/µL) 50 µL

Storage

–20°C

Control Template

In order to confirm that a sample does not include any inhibitory substances, perform a positive control experiment using the supplied control Template Set plus the sample template, and a reaction containing only sample template. In an inhibitor-free sample, bands derived from the Control Template (810 bp band with MCGp F1/R1 Primers, 590 bp with R2/R2 primers) appear in the positive control reaction; lack of bands in the control indicates the presence of an inhibitor.

Note: This product is optimized for use with TaKaRa Taq DNA Polymerase (not included). Use of Taq DNA Polymerase from another supplier may yield suboptimal results.

References

  1. Ueori, T. et al. (1992) System. Appl. Microbiol. 15:181-6.



 
 
Products
Cat. # Product Package Size Price # of Units Select
6601 PCR Mycoplasma Detection Set 100 Rxns $274.00
 

 

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