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Products >  Molecular_Biology >  Restriction_Enzymes >  Restriction_Enzymes_st >  Tth111I

Tth111I

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

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Features

DNA Restriction Enzyme: Tth111I

GACN|N NGTC
CTGN N|NCAG
  • Source: Thermus thermophiles strain 111
  • Concentration: 4-12 units/µl
  • Supplied buffer: K
  • Reaction temperature: 65°C
  • Substrate for unit definition: lambda DNA
  • Ligation-recutting test: The ligation efficiency of cohesive ended DNA fragments generated by this enzyme is low. More efficient ligation can be achieved using blunt end ligation reaction conditions.
  • Star activity: Cleavage at alternative site(s) occurs in the presence of Mn2+ and at alkaline pH. Known alternative cleavage sites include: NACNNNGTC, GNCNNNGTC, GANNNNGTC, GACNNNNTC, GACNNNGNC, and GACNNNGTN.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.




 
 
Products
Cat. # Product Contents Size Price Units Select
1090A Tth111 I 500 Units $225.00
1090B Tth111 I 2,500 Units $900.00
 

 

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