FeaturesDNA Restriction Enzyme: TaqI (TthHB8I)- Source: Thermus aquaticus YT-1
- Concentration: 4-12 units/µl
- Supplied buffer: Basal
- Reaction temperature: 65°C
- Substrate for unit definition: lambda DNA
- Effect of DNA methylation: Enzyme activity is affected by dam methylase depending on the sequence following the recognition site, but not affected by CG methylase.
- Star activity: Unrelated site may often be cut in the presence of high concentration of glycerol, and at alkaline pH or low ionic strength.
TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.
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