Takara
login | register | order now | view cart  
Products
Molecular Biology
Molecular Biology Supplies
Cloning, Mapping and cDNA Synthesis
DNA Labeling Kits
Gene Transfer and Expression
Instrumentation
Modifying Enzymes
Molecular Biology Reagents
Nucleic Acids
Plant Products
Restriction Enzymes
    Restriction Enzymes a-b
    Restriction Enzymes c-d
    Restriction Enzymes e-f
    Restriction Enzymes g-h
    Restriction Enzymes k-l
    Restriction Enzymes m-n
    Restriction Enzymes o-p
    Restriction Enzymes s-t
         Sac I
         Sac II
         Sal I
         Sau3A I (Mbo I)
         Sca I
         Sfi I
         Sma I
         Smi I (Swa I)
         SnaB I
         Spe I
         Sph I
         Sse 8387 I
         Ssp I
         Stu I
         Taq I (TthHB8 I)
         Tth111 I
    Restriction Enzymes u-v
    Restriction Enzymes w-z
RNA, Protein and DNA Extraction
Sequencing, Mutagenesis and Mutation


Products >  Molecular_Biology >  Restriction_Enzymes >  Restriction_Enzymes_st >  SphI

SphI

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

At-A-Glance Documents Images & Data

Features

DNA Restriction Enzyme: SphI

G CATG|C
C|GTAC G
  • Source: Streptomyces phaeochromogenes
  • Concentration: 4-12 units/µl
  • Supplied buffer: H
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Effect of DNA methylation: Enzyme activity is not affected by CG methylase.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.


 
 
Products
Cat. # Product Package Size Price # of Units Select
1246A Sph I 600 Units $122.00
1246B Sph I 3,000 Units $541.00
 

 

Recommended Products
Offers Cat. # Product Package Size Price License # of Units Select
R001A TaKaRa Taq™ DNA Polymerase 250 Units $118.00 License Statements
RR001A TaKaRa Ex Taq® DNA Polymerase 250 Units $164.00 License Statements
  3407A 100 bp DNA Ladder 50 ug $121.00  
RR310A EmeraldAmp® GT PCR Master Mix 160 Rxns $54.00  
 

 


Clontech is a Takara Bio Company © 2013 Privacy Policy | Terms & Conditions | TRUSTe