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Products >  Molecular_Biology >  Restriction_Enzymes >  Restriction_Enzymes_st >  SfiI

SfiI

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

At-A-Glance Documents Images & Data

Features

DNA Restriction Enzyme: SfiI

GGCCN NNN|NGGCC
CCGGN|NNN NCCGG
  • Source: Streptomyces fimbriatus
  • Concentration: 4-12 units/µl
  • Supplied buffer: M
  • Reaction temperature: 50°C
  • Substrate for unit definition: Ad2 DNA
  • Genome DNA analysis: Escherichia coli genomic DNA
  • Effect of DNA methylation: Enzyme activity is often affected by dcm methylase depending on the sequence of recognition site or the sequence following the site.
  • Star activity: Unrelated site may often be cut in the presence of Mn2+.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.


 
 
Products
Cat. # Product Package Size Price # of Units Select
1244A Sfi I 1,000 Units $179.00
1244B Sfi I 5,000 Units $780.00
 

 

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