Restriction enzyme NotI (Not I) | Isoschizomer of CciNI

Restriction Enzyme Overview

Restriction Enzymes a-b

Restriction Enzymes c-d

Restriction Enzymes e-f

Restriction Enzymes g-h

Restriction Enzymes k-l

Restriction Enzymes m-n

Mbo I (Sau3A I)

Mbo II

McrBC

Mfl I

Mlu I

Msp I (Hpa II)

Mun I (Mfe I)

Nae I

Nco I

Nde I

Nhe I

Not I

Nru I

Nsb I (Mst I, Avi II)

Restriction Enzymes o-p

Restriction Enzymes s-t

Restriction Enzymes u-v

Restriction Enzymes w-z

NotI

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

Features

DNA Restriction Enzyme: NotI

GGCC

CCGG

Source: Nocardia otitidis-caviarum

Concentration: 4-12 units/µl [High concentration: 30-60 units/µl]

Supplied buffer: H+BSA+Triton X-100

Reaction temperature: 37°C

Substrate for unit definition: Ad2 DNA

Genome DNA analysis: Escherichia coli genome DNA

Effect of DNA methylation: Enzyme activity is affected by CG methylase

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning


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NotI

Features

DNA Restriction Enzyme: NotI