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Products >  Molecular_Biology >  Restriction_Enzymes >  Restriction_Enzymes_mn >  McrBC

Methylation Dependent Restriction Enzyme McrBC

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

Methylation Dependent Restriction Enzyme

McrBC specifically cleaves DNA containing methyl cytosine (mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu : A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40 – 2,000 bp.  McrBC requires GTP for cleavage.

  At-A-Glance   Documents

Features

DNA Restriction Enzyme: McrBC

  • Source: recombinant proteins expressed in E. coli
  • Concentration: 1 unit /µl
  • Supplied buffer: McrBC
  • Reaction temperature: 37°C
  • Substrate for unit definition: pBR322
  • Effect of DNA methylation: This enzyme specifically cleaves DNA containing methyl cytosine (mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu : A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40 – 2,000 bp.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.

Applications

  • DNA methylation analysis (qAMP: quantitative analysis of DNA methylation using real-time PCR)
  • Enrichment of unmethylated DNA (CHARM: Comprehensive high-throughput arrays for relative methylation)

Components

10×McrBC Buffer 500 μL
0.1% BSA 1 mL
100 mM GTP (100×) 50 μL

Source

Recombinant protein expressed in E. coli




 
 
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1234A McrBC 200 Units $87.00
 

 

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