Restriction Enzyme EcoRI (EcoR I)

Restriction Enzyme Overview

Restriction Enzymes a-b

Restriction Enzymes c-d

Restriction Enzymes e-f

Eae I (Cfr I)

Eam1 105 I

Eco52 I (Xma III)

Eco81 I (Sau I)

EcoO109 I (Dra II)

EcoO65 I (Bst E II, BstP I)

EcoR I

EcoR V

EcoT14 I (Sty I)

EcoT22 I (Ava III)

Fba I (Bcl I)

Fok I

Restriction Enzymes g-h

Restriction Enzymes k-l

Restriction Enzymes m-n

Restriction Enzymes o-p

Restriction Enzymes s-t

Restriction Enzymes u-v

Restriction Enzymes w-z

EcoRI

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

Features

DNA Restriction Enzyme: EcoRI

AATT

TTAA

Source: Escherichia coli RY13

Concentration: 8-20 units/ul (High concentration: 30-60 units/µl)

Supplied buffer: H

Reaction temperature: 37°C

Substrate for unit definition: lambda DNA

Effect of DNA methylation: Enzyme activity is affected by CG methylase depending on the sequence following the recognition site.

Star activity: Unrelated site may often be cut in the presence of high concentration of glycerol or Mn²⁺, and at low ionic strength. Enzyme activity reduces to 70 to 80% by the addition of spermine (ca. 0.2mM), but star activity reduces to 30 to 50%.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning


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EcoRI

Features

DNA Restriction Enzyme: EcoRI