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Products >  Molecular_Biology >  Restriction_Enzymes >  Restriction_Enzymes_e_f >  Eco81I

Eco81I (SauI)

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

At-A-Glance Documents Images & Data

Features

DNA Restriction Enzyme: Eco81I (SauI)

CC|TNA GG
GG ANT|CC
  • Source: Escherichia coli RFL 8
  • Supplied buffer: M
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Ligation-recutting test: After digestion with this enzyme, ligation is inefficient due to the structure of the Eco81 I fragment ends (Janulaitis, A.A., personal communication). For circularized ligation, the overnight ligation using TaKaRa DNA Ligation Kit, Ver.1 or Ver.2.1 or Mighty Mix is recommended.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.


 
 
Products
Cat. # Product Package Size Price # of Units Select
1131A Eco81 I (Sau I) 500 Units $80.00
1131B Eco81 I (Sau I) 2,500 Units $315.00
 

 

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