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Restriction Enzymes
    Restriction Enzymes a-b
         Aat II
         Acc I
         Acc II (FnuD II)
         Acc III (BspM II)
         Afa I (Rsa I)
         Afl II
         Alu I
         Aor51H I (Eco47 III)
         Apa I
         ApaL I
         Bal I
         BamH I
         Ban II (HgiJ II)
         Bcn I (Cau II)
         Bgl I
         Bgl II
         Bln I (Avr II)
         BmeT110 I (Ava I)
         Bpu1102 I (Esp I)
         Bsp1286 I (Sdu I)
         Bsp1407 I
         BspT107 I (Hgi C I)
         BssH II (BseP I)
         Bst1107 I (Sna I)
         BstX I
    Restriction Enzymes c-d
    Restriction Enzymes e-f
    Restriction Enzymes g-h
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    Restriction Enzymes w-z
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Products >  Molecular_Biology >  Restriction_Enzymes >  Restriction_Enzymes_a-b >  BcnI

BcnI (CauII)

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

At-A-Glance Documents Images & Data

Features

DNA Restriction Enzyme: BcnI (CauII)

CC|(C/G) GG
GG (G/C)|CC
  • Source: Bacillus centrosporus RFL 1
  • Concentration: 10 units/µl
  • Supplied buffer: Basal
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Ligation-recutting test: After digestion with this enzyme, the ligation is inefficient under the influence of the structure of Bcn I fragments ends (Janulaitis,A.A., personal information). For circularized ligation, the overnight ligation using TaKaRa DNA Ligation Kit Ver.2.1 or Mighty Mix is recommended.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.


 
 
Products
Cat. # Product Package Size Price # of Units Select
1019A Bcn I (Cau II) 1,000 Units $80.00
1019B Bcn I (Cau II) 5,000 Units $315.00
 

 

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