FeaturesDNA Restriction Enzyme: BalI- Source: Brevibacterium albidum
- Concentration: 1-5 units/ul
- Supplied buffer: Basal
- Reaction temperature: 37°C
- Substrate for unit definition: lambda DNA
- Effect of DNA methylation: Enzyme activity is affected by dcm methylase depending on the sequence following the recognition site.
- Cleavage of pBR322: The commercially available pBR322 is substantially undigested because the cytosine residues in the recognition site (TGGCCA) are methylated by Dcm methylase
TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning
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