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Restriction Enzymes
    Restriction Enzymes a-b
         Aat II
         Acc I
         Acc II (FnuD II)
         Acc III (BspM II)
         Afa I (Rsa I)
         Afl II
         Alu I
         Aor51H I (Eco47 III)
         Apa I
         ApaL I
         Bal I
         BamH I
         Ban II (HgiJ II)
         Bcn I (Cau II)
         Bgl I
         Bgl II
         Bln I (Avr II)
         BmeT110 I (Ava I)
         Bpu1102 I (Esp I)
         Bsp1286 I (Sdu I)
         Bsp1407 I
         BspT107 I (Hgi C I)
         BssH II (BseP I)
         Bst1107 I (Sna I)
         BstX I
    Restriction Enzymes c-d
    Restriction Enzymes e-f
    Restriction Enzymes g-h
    Restriction Enzymes k-l
    Restriction Enzymes m-n
    Restriction Enzymes o-p
    Restriction Enzymes s-t
    Restriction Enzymes u-v
    Restriction Enzymes w-z
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Products >  Molecular_Biology >  Restriction_Enzymes >  Restriction_Enzymes_a-b >  ApaI

ApaI

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests.Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion.Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

At-A-Glance Documents Images & Data

Features

DNA Restriction Enzyme: ApaI

G GGCC|C
C|CCGG G
  • Source: Acetobacter pasteurianus sub. pasteurianus
  • Concentration: 8-20 units/ul; 30-60 units/ul (high concentration)
  • Supplied buffer: L
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Genome DNA Analysis: Staphylococcus aureus genome DNA
  • Effect of DNA methylation: Enzyme activity is affected by CG methylase
  • Definition of Activity: 3 units for lambda DNA as substrate is equivalent to 1 unit for an Ad2 substrate.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning


 
 
Products
Cat. # Product Package Size Price # of Units Select
1005A Apa I 10,000 Units $95.00
1005AH Apa I (High Concentration) 10,000 Units $95.00
1005B Apa I 50,000 Units $383.00
1005BH Apa I (High Concentration) 50,000 Units $383.00
 

 

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