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Products >  Molecular_Biology >  Restriction_Enzymes >  RestrictionEnzymeso_p >  PvuI

PvuI

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

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Features

DNA Restriction Enzyme: PvuI

CG AT|CG
GC|TA GC
  • Source: Proteus vulgaris
  • Concentration: 4-12 units/µl
  • Supplied buffer: K+BSA
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Genome DNA analysis: Flavobacterium okeanokoites genome DNA
  • Ligation-recutting test: The ligation efficiency of cohesive ended DNA fragments generated by this enzyme will be low. More efficient ligation can be achieved using blunt end ligation reaction conditions.
  • Effect of DNA methylation: Enzyme activity is affected by CG methylase, but not dam methylase.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
1242A Pvu I 400 Units $122.00
1242B Pvu I 2,000 Units $495.00
 

 

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