Restriction enzyme PstI (Pst I) | Isoschizomer of BstMAI

Restriction Enzyme Overview

Restriction Enzymes a-b

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PmaC I

PshA I

PshB I (Vsp I)

Psp1406 I (Acl I)

Pst I

Pvu I

Pvu II

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PstI

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

Features

DNA Restriction Enzyme: PstI

TGCA

ACGT

Source: Escherichia coli ED8654 carrying the plasmid encoding Pst I gene

Concentration: 8-20 units/µl [High concentration: 30-60 units/µl]

Supplied buffer: H

Reaction temperature: 37°C

Substrate for unit definition: lambda DNA

Star activity: Unrelated site may often be cut in the presence of high concentration of glycerol

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.


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PstI

Features

DNA Restriction Enzyme: PstI