TaKaRa DEXPAT Easy is designed for simple and rapid extraction of PCR-ready DNA from Formalin fixed paraffin-embedded (FFPE) tissues. This kit provides a ready-to-use optimized mixture of a special surfactant and PCR inhibitor-absorbent resin predispensed in 1.5-ml microtubes. Simply add paraffin-embedded tissue sections into one of the microtubes, incubate at 100°C, centrifuge at 4°C and cool on ice for 5 minutes to obtain PCR-ready DNA in approximately 30 minutes. The refrigerated centrifugation following heat treatment allows the formation of a gel-like layer above the absorbent resin. When collecting the DNA solution, the gel-like layer prevents contamination of the absorbent resin into the DNA solution. The absorbent resin may inhibit PCR amplification.
Extraction of DNA from paraffin-embedded tissue sections usually involves a very time-consuming and labor intensive process. TaKaRa DEXPAT Easy, however, offers a rapid and simplified procedure. This reagent retains the performance of TaKaRa DEXPAT, and has the added benefits of being predispensed in reaction tubes and has an improved composition designed to separate the DNA aqueous solution and the absorbent resin during DNA recovery.
