Yeast Processing Reagent (for Total RNA Preparation)
The Yeast Processing Reagent (for total RNA preparation) from Takara Bio is a pretreatment reagent that may be used in combination with RNA purification reagents to enable RNA isolation from yeast. Pretreatment of yeast cells with this product comprises two steps: 1) Washing and recovery of yeast cells by centrifugation, and 2) Yeast cell wall disruption via Yeast Processing Enzyme Solution (containing a cell wall degradation enzyme) and Yeast Processing Buffer. Because yeast cell wall disruption occurs through enzymatic activity, the time-consuming cell lysis protocols using glass beads and liquid nitrogen required in conventional methods are unnecessary.
