Protein and RNA Extraction Kit for Mammalian Cells
Storage
2–8°C
expression analysis, PAREx (Protein and RNA extraction Kit), RNAi effect
Components
Cell Lysis Buffer
1 x 25 mL
Denature Solution
3 x 25 mL
RNA Preparation Water
1 x 25 mL
Applications
Simultaneous extraction of total RNA and protein from cultured mammalian cells
RNAi effect and RNA and protein expression analysis
Features
Simple and quick protocol. A total RNA and protein solution can be prepared in about 15 minutes; a total RNA-only solution can be obtained with an additional isopropanol precipitation step in 50 minutes.
Simultaneous extraction of total RNA and protein from the same cellular material. Useful for RNAi effect analysis and RNA/protein expression analysis.
No solvents used (e.g. phenol/chloroform).
PAREx (Protein and RNA Extraction Kit) allows quick, simultaneous extraction of both total RNA and protein from cultured mammalian cells without the use of phenol or chloroform. Simultaneous extraction of total RNA and protein from the same sample simplifies protocol steps and is desirable for increased consistency in RNAi effect analyses and RNA and protein expression analysis applications.
PAREx uses a cell lysis buffer containing a nonionic surfactant for solubilizing cells and removes DNA and residual cells through a salting-out method. Preparation of the solubilized cell solution containing total RNA and protein, obtained from 1 x 103-1 x 107 cells, can be accomplished in approximately 15 minutes. This cell solution can be used without further preparation for electrophoretic migration and Western blot analysis of proteins. By further addition of isopropanol to the solution, RNA can be removed and then resolubilized in an RNase-free solution. This RNA solution is suitable for use in real-time RT-PCR reactions.
