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Products >  Molecular_Biology >  RNA_Protein_DNA_Extraction >  Gene_Trapping_by_Liquid_Extraction

Dr. GenTLE (Gene Trapping by Liquid Extraction) Systems

Takara's Dr. GenTLE (Gene Trapping by Liquid Extraction) Systems are designed for high quality and downstream application-ready DNA extraction and purification. Two Dr. GenTLE Systems are available: 1) Dr. GenTLE (from Yeast) High Recovery (Cat. # 9082) for efficient extraction of genomic DNA from yeast cells and 2) Dr. GenTLE Precipitation Carrier (Cat. # 9094) for high efficiency ethanol precipitation of nucleic acid solutions.

Dr. GenTLE (from Yeast) High Recovery includes reagents required for DNA extraction and purification from a variety of yeasts (see below) via cell wall digestion and cell lysis followed by salting. Genomic DNA isolated with this kit can be used for many applications including DNA sequencing, Southern blot analysis, cloning, PCR and restriction enzyme digestion. Most standard procedures using  2 x 108 yeast cells obtain 4–10 µg of genomic DNA (35–200 kb).

Dr. GenTLE Precipitation Carrier takes advantage of a high efficiency ethanol precipitation step during nucleic acid purification, resulting in higher DNA/RNA recovery even from dilute (5 ng/mL) nucleic acid samples versus glycogen-based precipitation. Dr. GenTLE Precipitation Carrier does not require a low temperature incubation step and produces an easily visualized white pellet, preventing nucleic acid loss during washing steps.

At-A-Glance Documents Images & Data

Applications

Dr. GenTLE (from Yeast) High Recovery

  • Quick and consistent yeast genomic DNA extraction in just 40 minutes
  • Downstream application-ready nucleic acids

Dr. GenTLE Precipitation Carrier

  • Precipitation of DNA/RNA from even dilute solutions (5 ng/ml nucleic acid)
  • Consistent, high-throughput nucleic acid preparation
  • DNA/RNA recovery from rare and unique samples

Components

Dr. GenTLE (from Yeast) High Recovery

Dr. GenTLE Yeast Solution A 15 mL
Dr. GenTLE Yeast Solution B3 mL
Dr. GenTLE Yeast Solution C6 mL
TE Buffer6 mL

Dr. GenTLE Precipitation Carrier

Dr. GenTLE Precipitation Carrier 1 x 800 µL
3M CH3COONa 2 x 1 mL
 

Dr. GenTLE (from Yeast) High Recovery Compatible Yeast

AYeast from which DNA can be preparedAshbya, Candida, Debaryomyces, Endomyces,
Eremothecium, Hanseniaspora, Hansenula, Kloeckera,
Kluyveromyces, Lipomyces, Metschikowia, Pullularia,
Saccharomyces, Saccharomycopsis, Saccharomycodes,
Schizosaccharomyces, Selenozyma, Trigonopsis,
Wickerhamia
BYeast from which DNA can be prepared
(Strain-dependent)
Bretanomyces, Cryptococcus, Nadsonia, Pichia,
Rodosporidium, Schwanniomyces,
Stephnoascus, Torulopsis
CYeast from which DNA cannot be preparedBullera, Pityrosporum, Rhosotorula, Sporidiobolus,
Sporobolomyces, Stetigmatomyces, Trichosporon

Storage

Dr. GenTLE (from Yeast) High Recovery: Store all components other than Dr. GenTLE Yeast Solution B at 4°C. Store Dr. GenTLE Yeast Solution B at room temperature as low temperature storage may result in formation of a precipitant. If a precipitate forms in Dr. GenTLE Yeast Solution B, dissolve by heating the solution at 37°C just before use.

Dr. GenTLE Precipitation Carrier: Room temperature.

 




 
 
Products
Cat. # Product Package Size Price # of Units Select
9082 Dr. GenTLE® (from Yeast) High Recovery 30 Rxns $123.00
9094 Dr. GenTLE® Precipitation Carrier 800 uL (200 Tests) $129.00
 

 


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