Takara's Dr. GenTLE (Gene Trapping by Liquid Extraction) Systems are designed for high quality and downstream application-ready DNA extraction and purification. Two Dr. GenTLE Systems are available: 1) Dr. GenTLE (from Yeast) High Recovery (Cat. # 9082) for efficient extraction of genomic DNA from yeast cells and 2) Dr. GenTLE Precipitation Carrier (Cat. # 9094) for high efficiency ethanol precipitation of nucleic acid solutions.
Dr. GenTLE (from Yeast) High Recovery includes reagents required for DNA extraction and purification from a variety of yeasts (see below) via cell wall digestion and cell lysis followed by salting. Genomic DNA isolated with this kit can be used for many applications including DNA sequencing, Southern blot analysis, cloning, PCR and restriction enzyme digestion. Most standard procedures using 2 x 108 yeast cells obtain 4–10 µg of genomic DNA (35–200 kb).
Dr. GenTLE Precipitation Carrier takes advantage of a high efficiency ethanol precipitation step during nucleic acid purification, resulting in higher DNA/RNA recovery even from dilute (5 ng/mL) nucleic acid samples versus glycogen-based precipitation. Dr. GenTLE Precipitation Carrier does not require a low temperature incubation step and produces an easily visualized white pellet, preventing nucleic acid loss during washing steps.
