The Deletion Kit for Kilo-Sequencing from Takara Bio is designed to facilitate DNA sequencing and especially sequencing of long DNA fragments cloned into the multiple cloning sites of M13 phage vectors (mp18/19) or pUC-related plasmid or phagemid vectors (pUC18/19). The Deletion Kit for Kilo-Sequencing can be used to create nested unidirectional deletions in target DNA, enabling progressive movement of the vector's primer binding site towards the sequence of interest. Optimized reagent cocktails are included in the Deletion Kit for Kilo-Sequencing to ensure efficient self-circularization of deletion subclones.
