This reagent is designed to enable one-step extraction of PCR-ready DNA from paraffin-embedded tissues fixed in 10% formalin. The one-step extraction protocol eliminates the laborious deparaffinization, organic solvent extraction and enzymatic digestion steps required in standard protocols.
DEXPAT Reagent preparation of PCR-ready DNA takes only 25 minutes, versus 2-3 days for conventional extraction methods.
Since DNA extracted from paraffin-embedded tissue may be somewhat degraded during formalin fixation or paraffin embedding, amplification of products greater than 400 bp in length is not recommended. The quality of the extracted DNA will be strongly dependent on the condition of the original embedded tissue.
