pRI 909/910 DNA are binary vectors which have a region of T-DNA for plant transformation. This originated from Ri plasmid of Rhizobium (Agrobacterium) rhizogenes, lacking their vir region. pRI 909/910 DNA are also shuttle vectors, and replicate autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, these vectors are high copy number plasmids because they have a replication origin the same as that of pUC type plasmid (ColE1 ori), and it is maintained stably in Rhizobium (Agrobacterium) also with mutant type replication origin of Ri plasmid (Ri-ori). These vectors contain the kanamycin resistant gene NPTIII for selection in E. coli and Rhizobium (Agrobacterium), and a mutant type kanamycin resistant gene NPTII for selection in plants.
The vectors are available for plant transformation in combination with Rhizobium (Agrobacterium) by binary vector system. It is possible to integrate a target gene in plant chromosome stably because the cloning site is located at the position closer to the Right Border(RB) of T-DNA than the selection marker (NPT II) for plant, so the target gene is not deleted.
