pRI 101 DNA series are binary vectors for expressing foreign genes in plant cells, which have the 35S promoter of caµliflower mosaic virus (CaMV) and a 5’non-coding region (5’-UTR) of alcohol dehydrogenase (ADH) gene. The 5’-UTR of ADH functions as a translational enhancer in plants. There are two types of vectors, pRI 101-AN DNA having a 5’-UTR of Arabidopsis ADH (AtADH 5’-UTR) and pRI 101 ON DNA having a 5’-UTR of rice ADH (OsADH 5’-UTR). The pRI 101-AN is for dicotyledonous plants such as tobacco or Arabidopsis and the pRI 101-ON is for monocotyledonous plants such as rice.
The pRI 101 DNA are shuttle vectors, and replicate autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, these vectors are high copy number plasmids because they have replication origins the same as the pUC type plasmids (ColE1 ori), and these are maintained stably in Rhizobium (Agrobacterium) also with mutant type replication origin of Ri plasmid (Ri-ori). The pRI 101 DNA are vectors capable of integrating target genes stably into plant chromosomes because the cloning site of these products is located at a position closer to the Right Border (RB) of T-DNA than the selection marker for the plant, so the target gene is not deleted.
