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Products >  Molecular_Biology >  Plant_Products >  Plant_DNA_Isolation_Reagent

Plant DNA Isolation Reagent

Takara plant DNA Isolation Reagent is a ready-to-use kit that includes benzyl chloride for extracting plant genomic DNA. Benzyl chloride causes disintegration of plant cell walls by benzylation of the hydroxyl groups in cellulose and other components of the cell wall. This kit takes advantage of this property in a procedure which involves freeze thawing, and then physical grinding of plant tissue with a pipette tip in a microcentrifuge tube to degrade the cell wall. Traditional methods of plant cell wall disruption, which rely on pulverization in liquid nitrogen using a mortar and pestle, are not needed. This makes preparing multiple samples at the same time more convenient. Furthermore, improvements in reagent composition have reduced the heat treatment to only 15 minutes, and the entire process through aqueous phase collection can be completed within 30 minutes. The genomic DNA obtained can be used directly for PCR or restriction endonuclease digestion.
At-A-Glance Documents

Features

  • Simple extraction of genomic DNA
  • No mortar and pestle needed to process plant samples
  • Ready-to-use kit
  • Isolation complete in 30 minutes

 

Components

Extraction Solution 140 mL
Extraction Solution 28 mL
Extraction Solution 3 (100% Benzyl Chloride)15 mL

Reagents required, but not provided in kit

Isopropanol
70% Ethanol
TE Buffer
RNase A (Optional)

Storage

Store at room temperature.
Storage of Extraction Solution 2 at a low temperature may cause a precipitate to form. This precipitate may be dissolved by heating to 50°C. Then store the solution at room temperature.


 
 
Products
Cat. # Product Package Size Price # of Units Select
9194 Plant DNA Isolation Reagent 100 Rxns $189.00
 

 


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