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Products >  Molecular_Biology >  Plant_Products >  Agrobacterium_tumefaciens_LBA4404_Electro-Cells

Agrobacterium tumefaciens LBA4404 Electro-Cells

Agrobacterium tumefaciens (Rhizobium radiobactor) can transfer T-DNA (transfer DNA), which is part of its own Ti plasmid, into host plant cells and insert this DNA into the plant chromosomal DNA. The inserted genes on T-DNA are expressed, then the cells are transformed into tumor cells called Crown gall. By utilizing this gene-transfer mechanism, the binary vector method was developed for plant transformation1. In this system, the pathogenic genes of T-DNA in Ti plasmid are replaced with selective marker genes and the exogenous target gene, permitting integration of the target gene into plant chromosomal DNA by means of Agrobacterium -mediated gene transfer.

Agrobacterium tumefaciens strain LBA4404 and the binary vector method was invented by Dr. P.J. Hooykaas at Leiden University in the Netherlands. This strain of Agrobacterium tumefaciens contains the pAL4404 plasmid, which only includes the T-DNA vir region (genes responsible for vir gene induction and T-DNA transfer). It is a widely used strain for plant transformation.

This product contains competent cells for transformation by electroporation3. Using A. tumefaciens and the binary vector method, you can transform various plants for infection (transfection) experiments.

At-A-Glance Documents

Features

  • High-quality electrocompetent cells: save time when performing your plant transformation experiments
  • Facilitates delivery of a binary vector 
  • Validated plasmid transformation efficiency 

Application

  • Prepare transgenic recombinant plants

Components

Agrobacterium tumefaciens LBA4404 Electro-Cells40 µL × 5 tubes
pRI900 DNA1 (1 ng/µl) 10 µL
SOC media2 1 mL × 10 tubes

1pRI900 DNA : pRI 910 DNA (Cat.# 3261) without a multicloning site
2SOC media: 2% Tryptone; 0.5% Yeast extract; 10 mM NaCl; 2.5 mM KCl; 10 mM MgSO4; 10 mM MgCl2; 20 mM Glucose

References

  1. A. Hoekema, P. R. Hirsch, P. J. J. Hooykaas., and R. A. Schilperoort., (1983) Nature, 303, 179-180.
  2. G. Ooms, P.J. J. Hooykaas, R. J. M. V. Veen, P. V. Beelen, T. J. G. Regensburg-Tuink, R. A. Schilperoort., (1982) Plasmid, 7, 15-29.
  3. S. Wen-jun and B. G. Forde (1989) Nucleic Acids Research, 17 (20), 8385

Quality

The transformation efficiency was tested with 1 ng of pRI900 DNA, and the colonies were selected in plates which included kanamycin and streptomycin. Efficiency of >5 x 106 colonies/µg of pRI 900 DNA was obtained. See Section "IV. Protocol" in the User Manual for more information.




 
 
Products
Cat. # Product Package Size Price # of Units Select
9115 Agrobacterium tumefaciens LBA4404 Electro-Cells 5 x 40 uL $225.00
 

 


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