Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Molecular Biology


Products >  Molecular_Biology >  Plant_Products >  Agrobacterium_tumefaciens_LBA4404_Electro-Cells

Agrobacterium tumefaciens LBA4404 Electrocompetent Cells

Agrobacterium tumefaciens (Rhizobium radiobactor) is capable of T-DNA transfer to plant cells. The T-DNA (transfer DNA) is located in the Ti plasmid and is capable of integration into the host plant chromosomal DNA. Integrated genes derived from T-DNA are expressed and the transformed plant cells typically become Crown gall tumor cells.

The binary vector method for plant transformationation1 takes advantage of Agrobacterium tumefaciens and its capacity to transfer T-DNA to plant cells. In this method, the pathogenic genes of Agrobacterium tumefaciens T-DNA in the Ti plasmid are replaced with selective marker genes as well as the exogenous target gene. This results in stable integration of the exogenous target gene into plant chromosomal DNA by means of Agrobacterium-mediated transformation. Agrobacterium tumefaciens strain LBA4404 and the binary vector method were invented by Dr. P.J. Hooykaas at Leiden University in the Netherlands. The LBA4044 strain of Agrobacterium tumefaciens contains the pAL4404 plasmid, which includes only the T-DNA vir region and enables only vir gene induction and T-DNA transfer. Agrobacterium tumefaciens Electrocompetent Cells LBA4044 are a widely used bacterial strain for T-DNA transfer to plant cells.

  At-A-Glance   Documents   Images & Data

Applications

  • Preparation of transgenic recombinant plants
  • T-DNA transfer to plant cells
  • Binary vector delivery
  • Highly efficient plasmid transformation

Components

Agrobacterium tumefaciens Electrocompetent Cells40 µL × 5 tubes
pRI900 DNA1 (1 ng/µl) 10 µL
SOC media2 1 mL × 10 tubes

1pRI900 DNA : pRI 910 DNA (Cat.# 3261) without a multiple cloning site
2SOC media: 2% Tryptone; 0.5% Yeast extract; 10 mM NaCl; 2.5 mM KCl; 10 mM MgSO4; 10 mM MgCl2; 20 mM Glucose

References

  1. Hoekema, A. et al. (1983) Nature 303:179-180.
  2. Ooms, G. et al. (1982) Plasmid 7:15-29.
  3. Wen-Jun, S. & Forde, B.G. (1989) Nucleic Acids Res. 17(20):8385.

Quality

Transformation efficiency was tested using 1 ng pRI900 DNA. Colonies were selected using kanamycin and streptomycin plates. Transformation efficiency of >5 x 106 colonies/µg of pRI900 DNA was achieved. See Section "IV. Protocol" in the User Manual for more information.




 
 
Products
Cat. # Product Contents Size Price Units Select
9115 Agrobacterium tumefaciens LBA4404 Electro-Cells 5 x 40 uL $230.00
 

 

 
 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions