pUC118/119 are plasmid vectors developed for the preparation of single stranded DNA. pUC118/119 was constructed by inserting intergenic region (IG region) of the M13 phage DNA into the pUC18/pUC19 plasmid.
Therefore, infection by the helper phage M13K07 induces the production of pUC118/pUC119 as single stranded DNA which is predominantly packaged into phage particles and is then released from bacterial cells. In addition, there is almost no contamination by the single stranded DNA of the helper phage. Using this system, single stranded DNA from large DNA fragments (up to 7 kb) can be stably obtained without deletion. pUC118 and pUC18 contains the consensus multi-cloning sites, samely pUC119 and pUC19 also.
