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Products >  Molecular_Biology >  Nucleic_Acids >  Vectors >  pUC_118-119_Vectors

pUC 118/119 Vectors

pUC118/119 are plasmid vectors developed for the preparation of single stranded DNA. pUC118/119 was constructed by inserting intergenic region (IG region) of the M13 phage DNA into the pUC18/pUC19 plasmid.

Therefore, infection by the helper phage M13K07 induces the production of pUC118/pUC119 as single stranded DNA which is predominantly packaged into phage particles and is then released from bacterial cells. In addition, there is almost no contamination by the single stranded DNA of the helper phage. Using this system, single stranded DNA from large DNA fragments (up to 7 kb) can be stably obtained without deletion. pUC118 and pUC18 contains the consensus multi-cloning sites, samely pUC119 and pUC19 also.

At-A-Glance Documents Images & Data

Features

  • Concentration: 250–1,000 µg/mL
  • Form: 10 mM Tris-HCI (pH8.0), 1 mM EDTA
  • Chain Length: 3,162 bp

Applications

  • Cloning of a target gene
  • DNA sequencing using M13 primers
  • Determination of long DNA sequences using Deletion Kit for Kilo-Sequencing (Cat.# 6030)
  • Gene expression using lac promoter

Purity

  • Double-strand covalently closed circular form I (RF I) comprises more than 70%.
  • It is confirmed that single-strand DNA is secreted upon infection of helper phage (M13KO7).
  • It is confirmed that the multi-cloning site is well-maintained upon sequence analysis by dideoxy method.

pUC118/119 DNA

  • It is confirmed that the vector contains a single cleavage site for EcoR I, Sac I, Kpn I, Sma I, BamH I, Xba I, Sal I, Pst I, Sph I and Hind III upon restriction analysis by the enzymes.

Storage

–20°C

GenBank

  Entry Name Accession No.
pUC118 U07649 U07649
pUC119 U07650 U07650



 
 
Products
Cat. # Product Package Size Price # of Units Select
3318 pUC 118 DNA 25 ug $47.00
3319 pUC 119 DNA 25 ug $47.00
 

 



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